Kinetics of Lymphocytic Subsets in Chicken Tracheal Lesions Infected with Infectious Bronchitis Virus

Journal of Veterinary Medical Science

Mitsuru KUWAMURA¹  Saeko WADA¹  Takao KOTANI¹  Yasuhiro TSUKAMOTO¹  Sadashige SAKUMA¹  yoji JYAMATE¹ 

[1] Department of Veterinary Pathology, College of Agriculture, Osaka Prefecture University, 1-1 Gakuencho, Sakai, Osaka 599-8531, Japan

关键词: B lymphocyte;    chicken;    infectious bronchitis virus;    T lymphocyte;    trachea;   

DOI  :  10.1292/jvms.62.397

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(19)Cited-By(11)The kinetics of T-cells (CD3 positive (+), CD4+ and CD8+ cells) and B-cells (IgG+, IgM+ and IgA+ cells) in chicken trachea were studied immunohistochemically and histopathologically following an intratracheal inoculation of infectious bronchitis virus (IBV). Viral antigen was detected in the cytoplasm of tracheal epithelium from 16 hr to 6 days post-inoculation (p.i.) with a peak on 4 days p.i. A few IgG+, IgM+ and IgA+ cells were detected in the submucosa from 8 hr p.i. Thereafter IgG+ and IgM+ cells were gradually increased in number, and dramatically increased from 3 days p.i., peaked on 4 days p.i., and gradually decreased after 5 days p.i. IgA+ cells were detected in a small number than IgG+ and IgM+ cells during the all experimental period. These B cells mainly existed in the lamina propria, and some cells were recognized in the interepithelial space. After 14 days p.i., small number of IgG+ and IgM+ cells were detected in the germinal center of lymph follicles in the lamina propria. From 24 to 60 hr p.i., a few number of CD3+, CD4+ and CD8+ cells were detected at the perivascular area in the lamina propria. After 3 or 4 days p.i., each positive T-cells increased rapidly in number, and reached on the peak at 5 days p.i. CD3+, CD4+ and CD8+ cells tend to distribute diffusely, perivascular area, and surrounding area of CD4+ cells, respectively. CD4+ cells were dramatically decreased from 7 days p.i., and CD3+ and CD8+ cells were decreased from 14 days p.i. No T-cells were detected in the lymph follicles in the lamina propria.

【 授权许可】

Unknown