Anti-tumor Effect of Polysaccharides From Scutellaria Barbata D. Don on the 95-D Xenograft Model via Inhibition of the C-Met Pathway

Journal of Pharmacological Sciences

Guihong Yang¹  Jinjin Wu¹  Yadong Yang¹  Xiaokun Yang¹  Qiaoyu Xu¹  Hui Tang¹  Shuqian Tang¹ 

[1] Department of Dermatology, Daping Hospital, Third Military Medical University, China

关键词: polysaccharides Scutellaria barbata (PSB);    c-Met;    95-D cell line;    lung cancer;    receptor tyrosine kinase;   

DOI  :  10.1254/jphs.13276FP

学科分类：药学

来源: Nihon Yakuri Gakkai Henshuubu / Japanese Pharmacological Society

【 摘 要 】

References(34)Cited-By(3)Polysaccharides isolated from Scutellaria barbata (PSB) have been reported to have anti-tumor effects. To investigate the underlying mechanism, a highly invasive, metastatic and phospho-c-Met overexpression lung carcinoma cell, 95-D cell line was used. The results showed that in vitro, PSB not only could inhibit the proliferation of 95-D cell line (IC50 = 35.2 μg/mL), but also down-regulated the expression of phospho-c-Met and its downstream signaling molecules including phospho-Erk and phospho-Akt. In vivo, PSB inhibited tumor growth in the 95-D subcutaneous xenograft model in a dose-dependent manner; after once-daily intraperitoneal injection for 3 weeks, tumor growth inhibition T/C ratio for 100 and 200 mg/kg treatments was 42.72% and 13.6%, respectively. In the end of the in vivo study, tumor tissues were harvested for further evaluation of the phosphorylation level of c-Met, AKT, and ERK. Ex vivo results demonstrated that the phosphorylation of c-Met and its downstream signaling molecules were also significantly inhibited by PSB. Immunohistochemistry analysis showed dose-dependent inhibition of tumor cell proliferation (Ki67) and reduction of microvessel density (CD31). In summary, the results indicated that PSB exerted anti-tumor growth activity on human lung cancer 95-D in vitro and in vivo by directly regulating the c-Met signaling pathway and the anti-tumor effects were mainly based on its anti-proliferation and anti-angiogenesis action.

【 授权许可】

Unknown