An Immunohistochemical Detection of Actin and Myosin in the Indigenous Bacteria-Adhering Sites of Microvillous Columnar Epithelial Cells in Peyer's Patches and Intestinal Villi in the Rat Jejunoileum

Journal of Veterinary Medical Science

Tetsurou INAMOTO²  Yuh YOKOO¹  Hidenori MIYATA¹  Kenkichi YAMAMOTO²  Wang-Mei QI²  Junichi KAWANO¹  Nobuhiko HOSHI¹  Hiroshi KITAGAWA¹  Toshifumi YOKOYAMA¹  Makiko NAMBA¹ 

[1] Department of Bioresource Science, Graduate School of Agricultural Science, Kobe University;Department of Bioresource and Agrobiosciences, Graduate School of Science and Technology, Kobe University

关键词: actin;    immunohistochemistry;    indigenous bacteria;    intestine;    myosin;   

DOI  :  10.1292/jvms.70.1153

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(28)Cited-By(8)The mechanism of physical elimination of indigenous bacteria was ultrastructurally and immunohistochemically investigated in microvillous columnar epithelial cells of Peyer's patches and intestinal villi of the rat jejunoileum. From ultrastructural observation, the microfilaments accumulated to form several electron-dense layers beneath the bacteria adhering to the cell membrane, which was slightly invaginated in the epithelial cells of Peyer's patches and intestinal villi. As the microfilamentous layers were forming, the end portions of invaginations were deformed into a cone-shape and were finally collapsed. At the same time, the end portions of the adhered bacteria were also deformed into cone-shapes. The bacterial cells were moved back toward the invagination orifices with no morphological change in their inner structure. From immunohistochemical observation, β-actin and nonmuscle-type myosin were detected at the thin layer just beneath the invaginated cell membrane. These findings suggest that indigenous bacteria which adhere to epithelial cells are removed by only a physical action of actin and myosin filaments, but are not killed. This bacterial cell removal system might lead to the establishment of a settlement of indigenous bacteria on host cells.

【 授权许可】

Unknown