Cloning of Feline cDNA Encoding the Cytotoxic T Lymphocyte-Associated Antigen 4 (CTLA-4)

Journal of Veterinary Medical Science

Koichi OHNO¹  Takafumi ONISHI¹  Miho FUJIKI¹  Hisashi INOKUMA¹  Tanvir S. KHATLANI¹ 

[1] Laboratory of Veterinary Internal Medicine. Faculty of Agriculture, Yamaguchi University, 1677-1 Yoshida, Yamaguchi 753-8515, Japan

关键词: CD28;    CTLA-4;    feline;   

DOI  :  10.1292/jvms.61.1241

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(19)Cytotoxic T lymphocyte-associated antigen 4 (CTLA-4) is a CD28 homologue which down-modulate T cell responses rather than augment them. To investigate its biological role in feline immune system, we cloned and sequenced full-length feline CTLA-4 (fCTLA-4) cDNA by RT-PCR from pokeweed mitogen stimulated peripheral blood lymphocytes. The fCTLA-4 contains an open reading frame of 669 nucleotides, coding for a polypeptide of 223 amino acids. The predicted fCTLA-4 amino acids sequence shows the homology of 86.6%, 87.0%, and 76.2% with human, bovine, and murine molecules respectively. The hexapeptide motif (MYPPPY) within the extra-cellular domain of CTLA-4 molecule, which is believed to be responsible for interaction with the B7 family members, is completely conserved in all the species.

【 授权许可】

Unknown