Development of a polymerase chain reaction diagnostic assay for Ceratomyxa shasta, a myxosporean parasite of salmonid fish

Diseases of Aquatic Organisms

Jerri L. Bartholomew¹  Oswaldo Palenzuela¹  Grant Trobridge¹ 

关键词: Myxozoa;    Diagnostics;    Ceratomyxa;    Polymerase chain reaction;    Ribosomal RNA gene;    rDNA;   

DOI  :  10.3354/dao036045

学科分类：生物科学（综合）

来源: Inter-Research

【 摘 要 】

ABSTRACT: A diagnostic procedure based on the polymerase chain reaction (PCR) was developed for the myxosporean parasite Ceratomyxa shasta. Three sets of oligonucleotide primers were designed to specifically amplify C. shastaribosomal RNA genes and several parameters of the assay were tested and optimised. A simple protocol for the processing of fish tissue samples was also developed. In a single round, 20 µl volume reaction the optimised procedure allows the detection of 50fg of purified C. shasta genomic DNA, or 0.01 spore from a seeded fish intestine sample. This protocol is considerably faster, cheaper and more reliable than any previous diagnostic procedure for a myxosporean parasite, and can be aninvaluable tool for the monitoring of early and/or subclinical C. shasta infections in wild and cultured salmon populations.

【 授权许可】

Unknown