An enzyme-linked immunosorbent assay for the detection of IgG antibodies against Babesia equi in horses

Ciência Rural

Massard, Carlos Luiz¹  Takakura, Felipe Santoro²  Botteon, Paulo de Tarso Landgraf¹  Machado, Rosangela Zacarias³  Baldani, Cristiane Divan² 

[1] Universidade Federal Rural do Rio de Janeiro;Universidade Estadual Paulista;Universidade Estadual Paulista, Jaboticabal

关键词: Babesia equi;    equine piroplasmosis;    ELISA;   

DOI  :  10.1590/S0103-84782004000500031

学科分类：农业科学（综合）

来源: Universidade Federal de Santa Maria * Centro de Ciencias Rurais

【 摘 要 】

A crude antigenic preparation of Babesia equi was used to develop and establish the suitability of an enzyme-linked immunosorbent assay (ELISA) for the detection of parasite carriers. Optimal dilutions of the antigen, using positive and negative reference sera, were determined by checkboard titrations. The specificity and sensitivity of the ELISA were 100 %. A total of 90 serum samples were taken from horses from the Northeast region of São Paulo State and examined for diagnosis of equine B. equi infection by ELISA. Approximately 75% (n=67) of all the horses tested were found serologically positive for B. equi. These results suggest that the ELISA described may prove to be an appropriate serological test for epidemiological studies on B. equi infections in the field and that equine piroplasmosis is a cause for serious concern in the State of São Paulo, Brazil.

【 授权许可】

CC BY-NC