Clinical trial results on the use of a recombinant feline interferon-ω to protect Japanese pearl oysters Pinctada fucata martensii from akoya- virus infection

Diseases of Aquatic Organisms

Naoki Nozawa¹  Tatsuya Kobayashi¹  Teruo Miyazaki¹ 

关键词: Japanese pearl oyster;    Akoya-virus infection;    Recombinant feline interferon-ω;    Efficacy in enhancement;    Agranulocyte;    Collagen fiber production;   

DOI  :  10.3354/dao043015

学科分类：生物科学（综合）

来源: Inter-Research

【 摘 要 】

ABSTRACT: Japanese pearl oysters (akoya oyster: Pinctada fucata martensii) are currently suffering mass mortalities from akoya-virus infection. In the present study, akoya oysters were injected with an anti-viral, recombinant felineinterferon-ω (rFeIFN-ω) in an attempt to confer resistance to this virus. In infectivity experiments, oysters were twice injected with rFeIFN-ω at 1 mega unit kg^-1of the meat weight. They were challenged with a single inoculation of cultured akoya-virus and held for 20 to 30 d at 25°C. Control oysters received only the viral challenge without rFeIFN-ω administration. In prophylacticexperiments, oysters that were given the akoya-virus on Days 1 to 5 after rFeIFN-ω administration showed lower mortalities. Furthermore, the survivors had fewer muscular lesions resulting from the virus infection than thecontrols. In treatment experiments, the virus was inoculated on Days 1 to 3 before rFeIFN-ω administration. None of the treated oysters died within a 30 d experimental period. Survivors displayed repaired lesions with fibroustissues that were produced by enhanced agranulocytes in the body musculature. Moreover, rFeIFN-ω was not toxic to akoya oysters. Thus, rFeIFN-ω administration is efficacious in preventing mortality ofakoya oysters with akoya-virus infection.

【 授权许可】

Unknown