Curcumin Induces Autophagy via Activating the AMPK Signaling Pathway in Lung Adenocarcinoma Cells

Journal of Pharmacological Sciences

Li Bai⁵  Jiayuan Sun⁵  Jiehan Jiang³  Chengping Hu²  Chunling Dong⁵  Guifang Wang⁵  Kui Xiao¹  Chaxiang Guan⁴  Chunxue Bai⁵ 

[1] Department of Respiratory Medicine, The Second Xiangya Hospital, Central South University, China;Department of Respiratory Medicine, Xiangya Hospital, Central South University, China;Department of Respiratory Medicine, Changsha Central Hospital, China;Department of Physiology, Xiangya School of Medicine, Central South University, China;Department of Pulmonary Medicine, Zhongshan Hospital, Fudan University, China

关键词: curcumin;    lung adenocarcinoma;    autophagy;    AMP-activated protein kinase (AMPK);   

DOI  :  10.1254/jphs.13085FP

学科分类：药学

来源: Nihon Yakuri Gakkai Henshuubu / Japanese Pharmacological Society

【 摘 要 】

References(57)Cited-By(19)Curcumin is a major yellow pigment and active component of turmeric widely used as dietary spice and herbal medicine. This compound has been reported to be a promising antitumor agent, although the underlying molecular mechanisms are not fully understood yet. In this study, we reported that curcumin inhibited growth of lung adenocarcinoma cells, but had no cytotoxic activity to IMR-90 normal lung fibroblast cells. Curcumin induced autophagy in the A549 human lung adenocarcinoma cell line, evidenced by LC3 immunofluorescence analysis and immunoblotting assays on LC3 and SQSTM1. Moreover, the autophagy inhibitor 3-MA partly blocked the inhibitory effect of curcumin on the growth of A549 cells. Curcumin markedly increased the phosphorylation of AMP-activated protein kinase (AMPK) and acetylCoA carboxylase in A549 cells. At last, pharmacological blockade of the AMPK signaling pathway by compound C and genetic disruption of the AMPK signaling pathway with siRNA-mediated AMPKα1 knockdown impaired the autophagy-inducing effect of curcumin. Collectively, our data suggests that curcumin induces autophagy via activating the AMPK signaling pathway and the autophagy is important for the inhibiting effect of curcumin in lung adenocarcinoma cells.

【 授权许可】

Unknown