Journal of Pharmacological Sciences | |
Curcumin Induces Autophagy via Activating the AMPK Signaling Pathway in Lung Adenocarcinoma Cells | |
Li Bai5  Jiayuan Sun5  Jiehan Jiang3  Chengping Hu2  Chunling Dong5  Guifang Wang5  Kui Xiao1  Chaxiang Guan4  Chunxue Bai5  | |
[1] Department of Respiratory Medicine, The Second Xiangya Hospital, Central South University, China;Department of Respiratory Medicine, Xiangya Hospital, Central South University, China;Department of Respiratory Medicine, Changsha Central Hospital, China;Department of Physiology, Xiangya School of Medicine, Central South University, China;Department of Pulmonary Medicine, Zhongshan Hospital, Fudan University, China | |
关键词: curcumin; lung adenocarcinoma; autophagy; AMP-activated protein kinase (AMPK); | |
DOI : 10.1254/jphs.13085FP | |
学科分类:药学 | |
来源: Nihon Yakuri Gakkai Henshuubu / Japanese Pharmacological Society | |
【 摘 要 】
References(57)Cited-By(19)Curcumin is a major yellow pigment and active component of turmeric widely used as dietary spice and herbal medicine. This compound has been reported to be a promising antitumor agent, although the underlying molecular mechanisms are not fully understood yet. In this study, we reported that curcumin inhibited growth of lung adenocarcinoma cells, but had no cytotoxic activity to IMR-90 normal lung fibroblast cells. Curcumin induced autophagy in the A549 human lung adenocarcinoma cell line, evidenced by LC3 immunofluorescence analysis and immunoblotting assays on LC3 and SQSTM1. Moreover, the autophagy inhibitor 3-MA partly blocked the inhibitory effect of curcumin on the growth of A549 cells. Curcumin markedly increased the phosphorylation of AMP-activated protein kinase (AMPK) and acetylCoA carboxylase in A549 cells. At last, pharmacological blockade of the AMPK signaling pathway by compound C and genetic disruption of the AMPK signaling pathway with siRNA-mediated AMPKα1 knockdown impaired the autophagy-inducing effect of curcumin. Collectively, our data suggests that curcumin induces autophagy via activating the AMPK signaling pathway and the autophagy is important for the inhibiting effect of curcumin in lung adenocarcinoma cells.
【 授权许可】
Unknown
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