Comparative Study of Anti-Oncogenic MicroRNA-145 in Canine and Human Malignant Melanoma

Journal of Veterinary Medical Science

Takayuki NAKAGAWA³  Nami YAMADA²  Shunsuke NOGUCHI²  Nobuo SASAKI³  Kohji MARUO⁴  Yuki HOSHINO⁴  Takashi MORI⁴  Yukihiro AKAO¹ 

[1] Comparative Cancer Center, Gifu University;The United Graduate School of Veterinary Sciences, Gifu University;Department of Veterinary Surgery, The University of Tokyo;Department of Veterinary Clinical Oncology, Gifu University

关键词: anti-oncogene;    c-MYC;    FASCIN1;    malignant melanoma;    microRNA-145;   

DOI  :  10.1292/jvms.11-0264

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(20)Cited-By(14)MicroRNA-145 (miRNA-145; miR-145) is aberrantly expressed in most of human cancers and plays a significant role in carcinogenesis and cancer progression. In the current study, we focused on how miR-145 plays a role in canine and human malignant melanomas. MiR-145 was significantly downregulated in canine malignant melanoma tissues and canine melanoma cell lines, as well as human melanoma cell lines tested. The ectopic expression of miR-145 showed a significant growth inhibition in both canine and human melanoma cells tested, and the effect was achieved partly by suppressing c-MYC in canine melanoma LMeC and in human melanoma A2058 and Mewo cells. At the same time, a suppressive tendency on cell migration in canine melanoma KMeC cells and significant suppression of cell migration in human melanoma A2058 cells by suppressing FASCIN1 were also found. These findings suggest that miR-145 acts as a tumor suppressor in both canine and human malignant melanomas.

【 授权许可】

Unknown