Journal of Veterinary Medical Science | |
Proteomics Analysis of Porcine Serum Proteins by LC-MS/MS after Foot-and-Mouth Disease Virus (FMDV) Infection | |
Youjun SHANG1  Jijun HE1  Yongjie LIU1  Ye JIN1  Xiangtao LIU1  Xuepeng CAI1  Haixue ZHENG1  Keshan ZHANG1  Guodong LU1  Jianhong GUO1  Hong TIAN1  | |
[1] State Key Laboratory of Veterinary Etiological Biology, National Foot and Mouth Disease Reference Laboratory, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences | |
关键词: foot and mouth disease virus infection; liquid chromatography-tandem mass spectrometry; porcine serum; proteomics analysis; | |
DOI : 10.1292/jvms.11-0019 | |
学科分类:兽医学 | |
来源: Japanese Society of Veterinary Science | |
【 摘 要 】
References(25)Cited-By(2)To analyze serum proteomics differences between normal and foot and mouth disease virus (FMDV)-infected piglets, an analytical method based on liquid chromatography with tandem mass spectrometry (LC-MS/MS) was used. Samples of venous blood were collected before and after FMDV infection and high abundance serum albumin was removed using a commercial kit. After trypsin digestion, serum samples were processed with LC-MS/MS. Proteins were identified by peptide mass fingerprinting. We found that apolipoprotein A-IV precursor, haptoglobin and probable chemoreceptor glutamine deamidase cheD appeared after FMDV infection in the same piglet. This is believed to be the first time that serum proteomics analysis by LC-MS/MS after FMDV infection has been performed, and our results may provide further information about biomarkers for early diagnosis of FMD in piglets.
【 授权许可】
Unknown
【 预 览 】
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