Proteomics Analysis of Porcine Serum Proteins by LC-MS/MS after Foot-and-Mouth Disease Virus (FMDV) Infection

Journal of Veterinary Medical Science

Youjun SHANG¹  Jijun HE¹  Yongjie LIU¹  Ye JIN¹  Xiangtao LIU¹  Xuepeng CAI¹  Haixue ZHENG¹  Keshan ZHANG¹  Guodong LU¹  Jianhong GUO¹  Hong TIAN¹ 

[1] State Key Laboratory of Veterinary Etiological Biology, National Foot and Mouth Disease Reference Laboratory, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences

关键词: foot and mouth disease virus infection;    liquid chromatography-tandem mass spectrometry;    porcine serum;    proteomics analysis;   

DOI  :  10.1292/jvms.11-0019

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(25)Cited-By(2)To analyze serum proteomics differences between normal and foot and mouth disease virus (FMDV)-infected piglets, an analytical method based on liquid chromatography with tandem mass spectrometry (LC-MS/MS) was used. Samples of venous blood were collected before and after FMDV infection and high abundance serum albumin was removed using a commercial kit. After trypsin digestion, serum samples were processed with LC-MS/MS. Proteins were identified by peptide mass fingerprinting. We found that apolipoprotein A-IV precursor, haptoglobin and probable chemoreceptor glutamine deamidase cheD appeared after FMDV infection in the same piglet. This is believed to be the first time that serum proteomics analysis by LC-MS/MS after FMDV infection has been performed, and our results may provide further information about biomarkers for early diagnosis of FMD in piglets.

【 授权许可】

Unknown