Passage of Chicken Egg Yolk Antibody Treated with Hydroxypropyl Methylcellulose Phthalate in the Gastrointestinal Tract of Calves

Journal of Veterinary Medical Science

Masahiko KUROKI¹  Masashi OHTA¹  Yutaka IKEMORI¹  Hideaki YOKOYAMA¹  Yoshikatsu KODAMA¹  Kouji UMEDA¹  Robert C. PERALTA¹ 

关键词: antibody;    gastrointestinal-tract;    HPMCP;   

DOI  :  10.1292/jvms.58.365

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(12)Cited-By(3)Two types of chicken egg yolk antibody samples for oral passage trials in calves were prepared; (1) hydroxypropyl methylcellulose phthalate (HPMCP) antibody powder (HAP) - a powder produced by spray-drying a supernatant obtained after precipitation of lipids from egg yolk with HPMCP and (2) control antibody powder (CAP) - a powder produced from an antibody solution without HPMCP. Antibody activity and pattern of distribution of both antibody preparations in the gastrointestinal tract of calves were compared by enzyme-linked immunosorbent assay. At 2 hr post administration, anti-K99 fimbrial antibodies from both the CAP and the HAP were detected in the abomasum of calves with titers of 1:128 and 1:256, respectively. However, at 4 hr, anti-K99 fimbrial titers of the CAP and the HAP were reduced to 1:2 and 1:64, respectively, due to digestion in the abomasum. These results indicated that the egg yolk antibody powder with HPMCP was more resistant against gastric juice in the stomach, thereby, ensuring a transfer of functional antibodies to the small intestine of calves after oral administration.

【 授权许可】

Unknown