Changes in Ascorbic Acid Content in Primary Cultured Rat Hepatocytes Exposed to 2,2'-Azobis (2-Amidinopropane) Dihydrochloride, a Radical Initiator

Journal of Veterinary Medical Science

Takehito ONOUE¹  Yosuke AOYAGI¹  Kazuhiro KIMURA¹  Keisuke SASAKI²  Hiroyuki OGURA¹  Yasunari KITAGUCHI¹ 

[1] Laboratory of Animal Nutrition, School of Agriculture, Ibaraki University;Department of Animal Products, National Institute of Livestock and Grassland Science

关键词: ascorbic acid;    oxidative stress;    rat hepatocyte;   

DOI  :  10.1292/jvms.63.925

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(12)Cited-By(1)Changes in ascorbate content in primary cultured rat hepatocytes exposed to oxidative stress derived from water soluble radical initiator 2,2'-azobis (2-amidinopropane) dihydrochloride (AAPH) were examined. Cells were exposed to 0.05 and 5 mg/ml of AAPH as `mild' and `severe' oxidative stresses, respectively. Lipid peroxidation in hepatocytes was induced by `severe' oxidative stress, but not by `mild' oxidative stress. Ascorbate decreased at 6 hr after administration of both `mild' and `severe' oxidative stresses, and recovered to the control level after a further 6 hr. In cells treated with `severe' oxidative stress, however, total ascorbate (reduced form plus oxidized form) had increased 24 hr after administration. These results indicated that consumption alone did not account for the increase of ascorbate in hepatocytes under oxidative stress.

【 授权许可】

Unknown