Use of Formalin-Fixed Paraffin-Embedded Tissue and Single-Strand Conformation Polymorphism Analysis for Polymerase Chain Reaction of Antigen Receptor Rearrangements in Dogs

Journal of Veterinary Medical Science

Toshiharu HAYASHI³  Masaru OKUDA⁴  Takashi TANIMOTO⁴  Takuya MIZUNO⁴  Takako SHIMOKAWA MIYAMA⁴  Naoki KANEKO⁴  Kazuhito ITAMOTO²  Hiroko HIRAOKA⁴  Satoshi UNE¹  Masahiro MORIMOTO³ 

[1] Laboratory of Veterinary Clinical Diagnosis, Faculty of Agriculture, Yamaguchi University;Laboratory of Veterinary Surgery, Faculty of Agriculture, Yamaguchi University;Laboratory of Veterinary Pathology, Faculty of Agriculture, Yamaguchi University;Laboratory of Veterinary Internal Medicine, Faculty of Agriculture, Yamaguchi University

关键词: cancer diagnosis;    canine;    lymphoma/leukemia;    PCR;    tumor diagnosis;   

DOI  :  10.1292/jvms.71.535

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(13)Cited-By(3)PCR for antigen receptor gene rearrangement analysis (PARR) is a new diagnostic method for lymphoid neoplasia. In PARR using formalin-fixed paraffin-embedded tissues (PARR-FFPE), control DNA amplification was successful in only three of five samples. The formalin fixation times of the three samples were shorter than those of the others. Analysis of the formalin fixation time and DNA amplification controls suggested that a formalin fixation time of less than one week is appropriate. Additionally, application of single strand conformation polymorphism (SSCP) for PARR provided clearer results than conventional PARR in 16 unfixed tissues and three FFPE tissues. These results show that PARR-FFPE is viable for tissues with an appropriate formalin fixation time and that application of FFPE and SSCP for PARR are useful for diagnosis and retrospective study of canine lymphoid neoplasia.

【 授权许可】

Unknown