Reduction of adhesion properties of Ruditapes philippinarum hemocytes exposed to Vibrio tapetis

Diseases of Aquatic Organisms

Christine Paillard¹  Philippe Soudant¹  Jean-Louis Nicolas¹  Christophe Lambert¹  Gwénaëlle Choquet¹ 

关键词: Cytotoxicity;    Flow cytometry;    Vibrio tapetis;    Ruditapes philippinarum;    Bivalve hemocytes;    Pathogenicity;   

DOI  :  10.3354/dao057109

学科分类：生物科学（综合）

来源: Inter-Research

【 摘 要 】

ABSTRACT: Vibrio tapetis is the causative agent of brown ring disease (BRD), which affects a species of clam, Ruditapes philippinarum. After incubation with V. tapetis, hemocytes lose filopods and become rounded, indicating cytotoxicactivity of the bacterium. To rapidly quantify this cytotoxicity, a flow-cytometry test was developed based on the capacity of V. tapetis to inhibit adhesion of clam hemocytes to plastic. Several bacteria:hemocyte ratios, the cytotoxicity of otherVibrio spp. pathogenic to bivalves, and that of various V. tapetis isolates were tested. Inhibition of adherence is detectable with as few as 5 bacteria per hemocyte. The greater cytotoxic activity of V. tapetis compared to that ofV. splendidus and V. pectenicida suggests a specific pathogenicity of V. tapetis to R. philippinarum hemocytes. Although all V. tapetis isolates inhibited adhesion, significant variations in cytotoxicity among isolateswas demonstrated.

【 授权许可】

Unknown