Baculovirus-Derived Hemagglutinin Vaccine Protects Chickens from Lethal Homologous Virus H5N1 Challenge

Journal of Veterinary Medical Science

C. Y. CHANG¹  M. S. CHIEN²  H. C. CHENG¹  S. H. WU¹  Y. L. CHEN¹  Y. J. LIN¹  C.C. HUANG¹  M. C. DENG¹  M. S. LEE¹ 

[1] Animal Health Research Institute, Council of Agriculture;Department of Veterinary Medicine, College of Veterinary Medicine, National Chung-Hsing University

关键词: baculovirus expression system;    H5N1;    hemagglutination inhibition;    highly pathogenic avian influenza (HPAI);    subunit vaccine;   

DOI  :  10.1292/jvms.70.1147

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(34)Cited-By(13)Since outbreaks of highly pathogenic avian influenza (HPAI) in both human and poultry from 2003, it is critical to have effective vaccines. A cDNA fragment coding the entire hemagglutinin (HA) gene derived from an H5N1 strain (A/duck/China/E319-2/03) was cloned and expressed using the baculovirus system. Two weeks after receiving two doses of recombinant HA (rHA) vaccines, chickens develop high antibody response for hemagglutination inhibition (HI) at titer 7.2 log2. Challenge studies revealed that vaccinated chickens with HI titers greater than 3 log2 could have immunoprotection against the same HPAI H5N1 strain virus challenge through intranasal route. Additionally, HI titer of 5 log2 determined whether the live viruses could not be detected from oropharyngeal, cloacal discharge or in tissues. This result suggests that the rHA expressed from baculovirus system could be a candidate for the development of a safe and efficient subunit vaccine for HPAI (H5N1).

【 授权许可】

Unknown