Effects of autologous adipose-derived stem cell infusion on type 2 diabetic rats

Endocrine Journal

Ruixia Sun³  Zhengju Fu³  Luan Wang³  Fang Wang³  Nina Tang¹  Jianxia Hu²  Ying Chen³  Shengli Yan³ 

[1] Nephrology Department, The Affiliated Hospital of Qingdao University, Qingdao 266003, China;Stem Cell Research Center, The Affiliated Hospital of Qingdao University, Qingdao 266003, China;Endocrinology Department, The Affiliated Hospital of Qingdao University, Qingdao 266003, China

关键词: Adipose-derived stem cells;    Type 2 diabetes mellitus;    Islet β cells;    Insulin resistance;   

DOI  :  10.1507/endocrj.EJ14-0584

学科分类：内分泌与代谢学

来源: Japan Endocrine Society

【 摘 要 】

References(40)The effects and possible mechanisms of adipose-derived stem cells (ASC) infusion on type 2 diabetic rats were investigated in this study.Twenty normal male Sprague-Dawley rats were included in normal control group, and 40 male diabetic rats were randomly divided into diabetic control group and ASC group (which received ASC infusion).After therapy, levels of fasting plasma glucose (FPG), HbA1c, serum insulin and C-peptide, recovery of islet cells, inflammatory cytokines, and insulin sensitivity were analyzed.After ASC infusion, compared with diabetic control group, hyperglycemia in ASC group was ameliorated in 2 weeks and maintained for about 6 weeks, and plasma concentrations of insulin and C-peptide were significantly improved (P<0.01).Number of islet β cells and concentration of vWF in islets in ASC group increased, while activity of caspase-3 in islets was reduced.Moreover, concentrations of TNF-α, IL-6 and IL-1β in ASC group obviously decreased (P<0.05).The expression of GLUT4, INSR, and phosphorylation of insulin signaling molecules in insulin target tissues were effectively improved.ASC infusion could aid in T2DM through recovery of islet β cells and improvement of insulin sensitivity.Autologous ASC infusion might be an effective method for T2DM.

【 授权许可】

Unknown