Cryptosporidium parvum in Korea: Prevalence in Individuals Residing in Three Major River Valleys and Genetic Characteristics of the Isolates

Journal of Veterinary Medical Science

Jae-Ran YU²  Tong-Soo KIM³  Yi-Young LIM²  Byoung-Kuk NA⁴  Isao KIMATA¹  Jung-Won JU²  Byung-Chul LEE²  Hyeng-Il CHEUN²  Jung-Yeon KIM²  Shin-Hyeong CHO² 

[1] Department of Medical Zoology, Graduate School of Medicine, Osaka City University;Division of Malaria and Parasitic Diseases, National Institute of Health, Korea Centers for Disease Control and Prevention;Department of Parasitology and Inha Research Institute for Medical Sciences, Inha University College of Medicine;Department of Parasitology and Institute of Health Sciences, Gyeongsang National University College of Medicine

关键词: Cryptosporidium parvum;    Korea;    PCR-RFLP;    River valley;   

DOI  :  10.1292/jvms.09-0296

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(30)Cited-By(4)Cryptosporidiosis is a diarrheal illness caused by apicomplexa parasite Cryptosporidium spp. In this study, to examine the overall infection status of Cryptosporidium spp. in individuals residing in southern parts of Korea, eight counties around Yeongsan, Seomjin and Nakdong River valleys was surveyed. The investigation was carried out from April to October 2005. A total of 9,498 stool samples were collected from individuals. Stool samples were analyzed for modified acid-fast stains, and DNA fragment extracted from positive samples was identified by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) for 18S rRNA polymorphic region. Oocysts of Cryptosporidium spp. were detected in 239 specimens (2.5%) by a modified acid-fast stain. Infection rate was not significantly different between male (2.2%) and female (2.8%) individuals examined (P>0.05). In the infection rate by age, totally 1-9 (4.8%) and 80< (3.7%) age group were shown to the highest, and there was shown to significant differences (P<0.05). Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis of 18S rRNA gene from 51 isolates showed that all the isolates were identified as C. parvum. Our data collectively suggested that C. parvum infection is prevalent in the studied areas of Korea and more comprehensive nation-wide epidemiological studies are needed to elucidate the infection status of Cryptosporidium infection in Korea.

【 授权许可】

Unknown