REMEDIAÇÃO DE PENTACLOROFENOL EMDOIS DIFERENTES TIPOS DE SOLO PORSphingomonas chlorophenolicaATCC 39723

Pesticidas: Revista de Ecotoxicologia e Meio Ambiente

Ken Killham¹  Rosemeri Inês Dams²  Graeme Paton¹ 

[1] University of Aberdeen - UK;Universidade do Vale do Itajaí

关键词: SOIL SYSTEMS;    PENTACHLOROPHENOL;    BIOAUGMENTATION;    PHYTOREMEDIATION;    Sphingomonas chlorophenolica;   

DOI  :  

学科分类：农业科学（综合）

来源: Universidade Federal do Parana * Centro de Pesquisa e Processamento de Alimentos

【 摘 要 】

The objective of this paper was study the Pentachlorophenol (PCP)degradation bySphingomonas chlorophenolicain two different types of soils: a loamy and a sandy soil in the presence and absence of plants (Winter wheat). Measurements of PCP concentrations were carried out in a laboratory basis using High performance liquid chromatography (HPLC). The toxic effect of PCP on plants was studied through the monitoring of plant weight and root length. The biodegradation of PCP byS. chlorophenolicain two different kinds of soil was assessed with a bioluminescence assay ofEscherichia coliHB101 pUCD607. Bacterial analyses were carried out by plating on three culture media: MSM (Mineral Salt Medium) forSphingomonas chlorophenolica , MSM for PCP_degrading/tolerant organisms and Trypticase Soy Broth Agar (TSBA,) for heterotrophic organisms. The introduction ofS. chlorophenolicainto the loamy soil with plants showed a faster degradation when compared to the inoculated soil without plants. The monitoring of the plant growth showed a protective role ofS. chlorophenolicaagainst the toxicity of PCP in the loamy soil. The bioassay confirmed that initial toxicity was lower while degradation progressed. In the sandy soil, there was no significant degradation of PCP and HPLC determination suggested that more than 75 % of PCP was sorbed into the soil. The presence of the inoculum did not significantly enhance the degradation of PCP. There was no  deleterious effect of PCP to the growth of winter wheat in this type of soil when plant weight and root length were measured. In both soils there was a significant increase of S. chlorophenolica, heterotrophic and PCPdegrading/ tolerant organisms in the roots when compared to those in the soil. This study showed that the presence of the inoculumS. chlorophenolicaenhanced the PCP degradation in a loamy soil and also it has a protective role to prevent phytotoxic effects of PCP on plant growth. The combined use of bioaugmentation and plants suggest that the rhizosphere of certain plant species may be important for facilitating microbial degradation of pesticides in soil with important implications for using vegetation to stabilize and remediate surface soils.

【 授权许可】

Unknown