Negative Inotropic Effect of Carbachol and Interaction between Acetylcholine Receptor-Operated Potassium Channel (K.ACh Channel) and GTP Binding Protein in Mouse Isolated Atrium �? A Novel Methodological Trial

Journal of Veterinary Medical Science

Chihiro NOMA¹  Hideyuki YAMAWAKI¹  Yukio HARA¹  Muneyoshi OKADA¹ 

[1] Laboratory of Veterinary Pharmacology, School of Veterinary Medicine, Kitasato University, Higashi 23 bancho 35-1, Towada city, Aomori 034-8628, Japan

关键词: K.ACh channel-G protein interaction;    mouse atrium;    negative inotropism;    tertiapin;   

DOI  :  10.1292/jvms.12-0369

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(21)Interaction between acetylcholine receptor-operated potassium channel (K.ACh channel) and GTP binding protein was examined by an immunoprecipitation-Western blotting system in mouse isolated atrium. The carbachol-induced negative inotropic action in indomethacin-pretreated mouse atrium was significantly inhibited by a K.ACh channel blocker, tertiapin or atropine. Kir3.1 K.ACh channel (Kir3.1) was immunoprecipitated with a mouse anti-Kir3.1 antibody. Coprecipitating Gβ with Kir3.1, detected by Western blotting, was significantly augmented by carbachol. Atropine, but not tertiapin, significantly inhibited the carbachol-induced coprecipitating Gβ with Kir3.1. The data indicate that immunoprecipitation with Kir3.1 and Western blotting of Gβ system is a useful method for assessing interaction between K.ACh channel and GTP binding protein in mouse atrium.

【 授权许可】

Unknown