Adenosine and ATP Affect LPS-Induced Cytokine Production in Canine Macrophage Cell Line DH82 Cells

Journal of Veterinary Medical Science

Yuka FUJIMOTO¹  Naoko NAKATANI¹  Tadayoshi TAKEUCHI²  Takeya KUBO²  Toshie ISERI¹  Hidemitsu NAKAJIMA²  Yuko SEMI²  Yasu-Taka AZUMA²  Natsuho YOSHIDA² 

[1] Laboratory of Advanced Diagnosis and Treatment, Division of Veterinary Science, Graduate School of Life and Environmental Science, Osaka Prefecture University;Laboratory of Veterinary Pharmacology, Division of Veterinary Science, Graduate School of Life and Environmental Science, Osaka Prefecture University

关键词: adenosine;    ATP;    cytokine;    LPS;    macrophage;   

DOI  :  10.1292/jvms.11-0228

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(36)Cited-By(3)Macrophages are essential for controlling the majority of infections, and are mediators of natural immunity. During infection, lipopolysaccharide (LPS) stimulates macrophages to produce pro-inflammatory cytokines. Adenosine and ATP released into the extracellular space by immunological stimuli have been shown to regulate various immune functions. More recently, it has been shown adenosine and ATP have a critical role on the physiological negative feedback mechanism for limitation and termination of tissue-specific and systemic inflammatory responses. It was useful and meaningful to gain information about interaction between LPS, which generates the inflammation, and adenosine and ATP, which terminate the inflammation. We evaluate effects of adenosine and ATP on the production of cytokines related to inflammation in canine macrophage cell line DH82 cells. Adenosine and ATP respectively increased the production of IL-10 without affecting the production of IL-6, TNF-α and IL-12 in DH82 cells. In addition, adenosine and ATP prevented the production of LPS-induced IL-6, TNF-α and IL-12 in DH82 cells. In contrast, adenosine and ATP potentiated LPS-induced IL-10 production in DH82 cells. Moreover, adenosine, but not ATP inhibited LPS-induced expression of TLR4 in DH82 cells. These results suggest that conditions related to increased adenosine and/or ATP may play an important role in the inflammatory reactions.

【 授权许可】

Unknown