Detection and Molecular Typing of Human Adenoviruses Associated with Respiratory Illnesses in Kerala

Japanese Journal of Infectious Diseases

Amol Baburao Mun¹  Asia Devi Thounaojam¹  Anukumar Balakrishnan¹ 

[1] National Institute of Virology (Kerala Unit), Government Thirumala Dewaswom Medical College (TDMC) Hospital

关键词: human adenovirus;    respiratory illness;    Kerala;    HAdV-E4;    HAdV-C2;   

DOI  :  10.7883/yoken.JJID.2015.414

学科分类：传染病学

来源: National Institute of Infectious Diseases

【 摘 要 】

References(33)Adenoviruses are responsible for approximately 5–10% of acute respiratory infections globally. However, there are a limited number of reports on the types of circulating respiratory human adenoviruses (HAdV) in India. We detected HAdV in the post-mortem specimens of a young child who died as a result of an acute febrile illness. To retrospectively investigate the circulating adenovirus types in the Alappuzha region, samples (n = 235) collected from patients with influenza-like illnesses who participated in the influenza surveillance program were screened for HAdV. Fourteen samples were identified as positive for adenovirus by PCR analysis. Adenovirus was isolated from 3 of the 14 PCR-positive samples cultured using HEK-293 cell lines. The viral strains isolated in the study were from children between 6 and 10 years of age. The isolates were identified as adenovirus species C and E. Sequencing analysis of the fiber gene and a BLAST search revealed that 2 of the isolates were type HAdV-C2, and the third isolate was a HAdV-E4. A fiber gene sequence-based phylogenetic tree showed that the HAdV-E4 isolate was similar to the Japanese HAdV-E4 strain, whereas the HAdV-C2 isolates formed a distinct cluster. Respiratory infections due to HAdV-E4 are generally observed in adults; this study is the first to demonstrate the involvement of the HAdV-E4 strain in respiratory illnesses in children.

【 授权许可】

Unknown