One-Step Immunochromatography Assay for Detection of High-Level Canine Serum Trypsin-Like Immunoreactivity

Journal of Veterinary Medical Science

Mitsuru FURUICHI¹  Masaharu HISASUE¹  Ryo TSUCHIYA¹  Yoko OKUNO²  Masaaki ONDA²  Takaki WARITANI²  Sakurako NEO¹  Takatsugu YAMADA¹ 

[1] Laboratory of Veterinary Internal Medicine, School of Veterinary Medicine, Azabu University;Animal Health Products and Chemicals Division, Animal Health Products and Chemicals Research Section, Daiichi Fine Chemical Co., Ltd.

关键词: canine;    pancreatitis;    TLI;   

DOI  :  10.1292/jvms.69.669

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(18)Cited-By(1)We developed a one-step immunochromatography assay kit to measure high levels of canine trypsin-like immunoreactivity (cTLI) for bedside estimation of canine pancreatitis. The serum cTLI level can be determined within 10 min by visual comparison of color strengths in the test and reference zones. The serum cTLI levels determined by this method correlate well with canine TLI-ELISA and can be classified into 3 categories: cTLI levels higher than 60 ng/ml were considered positive; 20-60 ng/ml, weakly positive; and less than 20 ng/ml, negative. Twelve dogs suspected of pancreatitis were examined using this method; 4 dogs were positive, 2 were weakly positive, and 6 were negative. This test can detect a high level of serum cTLI and a positive result in the TLIH test will provide critical information for evaluation of pancreatitis in dogs.

【 授权许可】

Unknown