Molecular varibility of Venezuelan isolates of entomopathogenic nematodes and their symbiont bacteria

Revista de Protecci贸n Vegetal

Enrique, Roberto²  Peteira, Belkis²  Rodríguez, Mayra G.²  Salazar, Efraín¹  Rosales, Carolina¹  Casado, Raúl¹  Maselli, Anna¹  Miranda, Ileana²  Castro, Luis¹ 

[1] Instituto Nacional de Investigaciones Agrícolas (INIA), Maracay, Venezuela;Centro Nacional de Sanidad Agropecuaria (CENSA), Mayabeque, Cuba

关键词: entomopathogenic nematodes;    RAPD;    Molecular variability;   

DOI  :  

学科分类：农业科学（综合）

来源: Centro Nacional de Sanidad Agropecuaria

【 摘 要 】

The aim of this study was to determine the molecular variability in a collection of Venezuelan isolates of entomopathogenic nematodes and their symbiont bacteria through the application of RAPD markers. The DNA of nematodes and bacteria were extracted by a modification of the method described by Salazar et al. RAPD assay was developed with the primers from the kits OPA and OPB. Genetic fingerprinting of isolates of the nematode and symbiotic bacteria were determined. The total amplified bands for nematode was 493 and 496 for bacteria, with 99,18 and 100% polymorphism, respectively. The clustering obtained for the nematodes was related to the altitude above the sea level at which they were collected, while for the bacteria, the clustering was in general related to the type of soil from which they came.

【 授权许可】

Unknown