Involvement of Mitochondrial Genes of Babesia gibsoni in Resistance to Diminazene Aceturate

Journal of Veterinary Medical Science

Masahiro YAMASAKI¹  Yu TAMURA¹  Sue Yee LIM¹  Mitsuyoshi TAKIGUCHI¹  Masahiro MURAKAMI¹  Shiang-Jyi HWANG¹  Bandula Kumara WICKRAMASEKARA RAJAPAKSHAGE¹  Noboru SASAKI¹  Hiroshi OHTA¹  Kensuke NAKAMURA¹ 

[1] Laboratory of Veterinary Internal Medicine, Department of Veterinary Clinical Sciences, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo 060–0818, Japan

关键词: Babesia gibsoni;    cytochrome b;    cytochrome c oxidase I;    cytochrome c oxidase III;    diminazene aceturate-resistant isolate;   

DOI  :  10.1292/jvms.12-0056

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(34)Cited-By(3)The stability of the characteristics of the diminazene aceturate (DA)-resistant B. gibsoni isolate was initially determined invitro. Part of the DA-resistant B. gibsoni isolate was cultured without DA for 4 weeks, and then newly exposed to 200 ng/ml DA. As a result, this isolate could proliferate the same as the DA-resistant isolate, indicating that the characteristic of DA resistance was stable in the DA-resistant isolate. Additionally, the level of parasitemia in the DA-resistant isolate was comparatively lower than in the wild-type, suggesting that the proliferation potential of the DA-resistant isolate would be lower than that of the wild-type. Subsequently, to investigate the involvement of mitochondrial DNA (mtDNA) in DA resistance in B. gibsoni, the nucleotide sequences and deduced amino acid sequences of mitochondrial genes such as COXI, COXIII, and CYTb genes of the DA-resistant isolate, were compared with those of the wild-type. As a result, these three genes were not altered in the DA-resistant B. gibsoni isolate. Moreover, the transcription levels of COXI, COXIII, and CYTb genes were observed by semi-quantitative RT-PCR. As a result, the gene transcription of those genes in the DA-resistant isolate was not significantly altered. These results indicated that DA did not affect mtDNA directly in DA-resistant B. gibsoni. Thus, it is suggested that mtDNA should not be deeply involved in DA resistance in B. gibsoni.

【 授权许可】

Unknown