Clonal Endothelial Cells Produce Humoral Factors that Inhibit Osteoclast-Like Cell Formation In Vitro

Endocrine Journal

KAZUKI YANO¹  TAKASHI TSURUO³  TOSHIRO FUJITA²  NORIKO CHIKATSU²  NAOYA FUJITA³  SEIJI FUKUMOTO⁴  YASUHIRO TAKEUCHI² 

[1] Research Institute of Life Science, Snow Brand Milk Products Co., Ltd.;Division of Endocrinology Department of Medicine, University of Tokyo School of Medicine;Institute of Molecular and Cellular Biosciences, University of Tokyo;Department of Laboratory Medicine, University of Tokyo School of Medicine

关键词: Endothelial cell;    Bone marrow cell;    Osteoclast;    RANKL;    Osteoprotegerin;   

DOI  :  10.1507/endocrj.49.439

学科分类：内分泌与代谢学

来源: Japan Endocrine Society

【 摘 要 】

References(40)Cited-By(8)Angiogenesis and bone remodeling are closely associated, and vascular endothelial cells may have potential roles for osteoclastic bone resorption. We examined whether clonal endothelial cells established from bone, aorta and brain of Balb/c mice influenced osteoclast-like cell formation in vitro. As low as 1% conditioned media of those endothelial cells inhibited osteoclast-like cell formation in bone marrow cultures induced by 1,25-dihydroxyvitamin D3, and did so in spleen cell cultures in the presence of soluble receptor activator of nuclear factor-κB ligand (RANKL), M-CSF and prostaglandin E2. The level of osteoprotegerin (OPG), a decoy receptor for RANKL, secreted by endothelial cells was not high enough to inhibit osteoclastogenesis. These observations suggest that endothelial cells derived from various tissues secrete factor(s) that inhibits precursors to differentiate into osteoclasts even in the presence of optimal stimulators for osteoclastogenesis. Hence, endothelial cells in bone may inhibit recruitment of fresh osteoclasts, and those in tissues other than bone may be involved in prohibiting ectopic osteoclastogenesis.

【 授权许可】

Unknown