The Role of a New Liquid-Solid Scintillator, a Mixture of DPO, POPOP and Epon 812, for Enhancement of Autoradiograms with Tritium Agents

Journal of Veterinary Medical Science

Toshiyuki NISHIMAKI²  Sen-ichi FURUDATE¹ 

[1] Laboratory Animal Science, Kitasato University School of Medicine;Department of Radioisotope Center, Kitasato University School of Medicine

关键词: autoradiography;    enhancer;    3H;    liquid-solid scintillator;    tumor;   

DOI  :  10.1292/jvms.60.795

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(11)It is desirable to shorten expose time in 3H-autoradiography. We developed a new solid scintillator, a mixture of DPO, POPOP and Epon 812, for whole-body autoradiography. The new scintillator is originally in a sol form, but quickly transforms to a solid homogeneous film following exposure to room air. The new scintillator enabled whole-body autoradiography to be developed faster when compared with a liquid scintillator or commercially available reagents. We also demonstrated that the new scintillator gave consistently high counting performance and thus shortened duration of exposure when used in whole-body autoradiography, whereas commercially available enhancers were found to be unsuitable for autoradiography, resulting in dappled images. Furthermore the quantitative analyses of the autoradiograms by a densitometer demonstrated the highest density, and ratio with this new solid scintillator among those with a liquid scintillator and commercially available reagents. In conclusion, a new solid scintillator, a mixture of DPO, POPOP and Epon 812, is a useful and cost effective enhancer for the whole-body autoradiography of tritum compounds.

【 授权许可】

Unknown