Osthole Augments Therapeutic Efficiency of Neural Stem Cells–Based Therapy in Experimental Autoimmune Encephalomyelitis

Journal of Pharmacological Sciences

Jingxian Yang²  Peng Gao³  Zhong Gao⁴  Yang Xia¹  Hongyan Li²  Safeng Zou⁴  Nan Zhang²  Qingping Wen³ 

[1] Department of Engineering, St. Cross College, University of Oxford, UK;School of Pharmacy, Liaoning University of Traditional Chinese Medicine, China;Department of Anesthesiology, First Affiliated Hospital, Dalian Medical University, China;Department of Interventional Therapy, Department of Rehabilitation, Dalian Municipal Central Hospital, China

关键词: neural stem cell;    experimental autoimmune encephalomyelitis (EAE);    osthole;    anti-inflammation;    remyelination;   

DOI  :  10.1254/jphs.13144FP

学科分类：药学

来源: Nihon Yakuri Gakkai Henshuubu / Japanese Pharmacological Society

【 摘 要 】

References(38)Cited-By(14)The therapeutic potential of adult neural stem cells (NSCs)-derived from bone marrow (BM) has been recently described in experimental autoimmune encephalomyelitis (EAE), an animal model of multiple sclerosis; however, the beneficial effects are modest due to their marginal anti-inflammatory capacity. To overcome this weakness and endow BM-NSC therapy with profound anti-inflammatory capacity, in this study we pretreated EAE mice with osthole, a natural coumarin with a broad spectrum of pharmacological activities, including anti-inflammation, immunomodulation, and neuroprotection, before NSC-application and continued throughout the study. We found that osthole conferred a potent anti-inflammatory capacity to this BM-NSC therapy, thus more profoundly suppressing ongoing EA and exhibiting significant advantages over conventional NSC-therapy as follows: 1) Enhanced anti-inflammatory effect, thus improving survival environment for engrafted BM-NSCs and protecting myelin sheaths from further demyelination; 2)Drove transplanted (exogenous) BM-NSCs to differentiate into more oligodendrocytes and neurons but inhibited differentiation into astrocytes, thus promoting remyelination and axonal growth, and reducing astrogliosis; and 3) augmented CNS neurotrophic support thus promoted resident (endogenous) repair of myelin/axonal damage. These effects make the BM-NSCs–based therapy a more promising approach to enhance remyelination and neuronal repopulation, thus more effectively promoting anatomic and functional recovery from neurological deficits.

【 授权许可】

Unknown