A Genetic Method for Sex Identification of Raccoons (Procyonlotor) with Using the ZFX andZFY Genes

Journal of Veterinary Medical Science

Toshio TSUBOTA¹  Minami W. OKUYAMA¹  Michito SHIMOZURU¹ 

[1] Laboratory of Wildlife Biology and Medicine, Graduate School of Veterinary Medicine, Hokkaido University, Kita18, Nichi9, Kita-ku, Sapporo, Hokkaido 060�?0818, Japan

关键词: PCR;    raccoons;    sex identification;    ZFX and ZFY genes;   

DOI  :  10.1292/jvms.13-0577

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(8)A genetic method for sex determination in raccoons was developed based onnucleotide differences of the zinc finger protein genes ZFX andZFY. Four novel internal primers specific for ZFX orZFY were designed. PCR amplification using two primer sets followed byagarose gel electrophoresis enabled sex determination. 141-bp and 447-bp bands were inboth sex, and 346-bp band was specific only in male with primer set I. 345-bp and 447-bpbands were in both sex, and 141-bp band was specific only in male with primer set II,which could distinguish raccoon’s electrophoresis pattern from three native carnivores inHokkaido. This method will be useful for conservation genetics studies or biologicalanalyses of raccoons.

【 授权许可】

Unknown