cDNA Sequence and Tissue Distribution of Canine Na-Dependent Neutral Amino Acid Transporter 2 (ASCT 2)

Journal of Veterinary Medical Science

Ken ONDA³  Hideharu OCHIAI¹  Kikumi OGIHARA⁴  Takuya MARUO²  Yuko NAYA⁴  Hiroki SUGIYAMA² 

[1] Research Institute of Biosciences, Azabu University, 1–17–71 Fuchinobe, Chuo-ku, Sagamihara, Kanagawa 252–5201, Japan;Veterinary Teaching Hospital, Azabu University, 1–17–71 Fuchinobe, Chuo-ku, Sagamihara, Kanagawa 252–5201, Japan;Laboratory of Internal Medicine 3, Azabu University, 1–17–71 Fuchinobe, Chuo-ku, Sagamihara, Kanagawa 252–5201, Japan;Laboratory of Pathology, Azabu University, 1–17–71 Fuchinobe, Chuo-ku, Sagamihara, Kanagawa 252–5201, Japan

关键词: ASCT2;    canine;    cDNA sequence;    distribution;    SLC1A5;   

DOI  :  10.1292/jvms.12-0171

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(24)Cited-By(1)A full-length cDNA sequence of canine Na-dependent neutral amino acid transporter (ASCT2) and its distribution were determined. The sequence was 2,090 bp long and was predicted to encode 544 amino acid polypeptides. The amino acid sequence deduced from canine ASCT2 showed 90% similarity to that of humans and mice. Northern blot analysis revealed ASCT2 expression in the kidney, heart, lung and muscles, and Western blot analysis using anti-human ASCT2 antiserum detected the bands at 60 and 65 kDa in membrane protein of the lung. RT-PCR analysis revealed ASCT2 expression in all the tissues examined.

【 授权许可】

Unknown