The Effects of Twisting and Type of Aspiration Needle on the Efficiency of Transvaginal Ultrasound-Guided Ovum Pick-Up in Cattle

Journal of Veterinary Medical Science

Yoshiyuki TAKAHASHI²  Yoshihiko SASAMOTO²  Minoru SAKAGUCHI¹  Yutaka YAMADA¹  Seiji KATAGIRI² 

[1] Laboratory of Animal Physiology and Reproduction, Department of Animal Production, National Agricultural Research Center for Hokkaido Region;Laboratory of Theriogenology, Department of Veterinary Clinical Sciences, Graduate School of Veterinary Medicine, Hokkaido University

关键词: cattle;    needle twisting;    needle type;    oocyte;    ovum pick-up;   

DOI  :  10.1292/jvms.65.1083

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(19)Cited-By(3)The effects of twisting and type (single- or double-lumen) of aspiration needle on the efficiency of transvaginal ultrasound-guided ovum pick-up (US-guided OPU) were investigated in cattle. The first study using slaughterhouse ovaries revealed that twisting of the needle during follicle aspiration improved the oocyte recovery rate without deleterious effects on the attachment of cumulus layers. Vacuum pressure affected the oocyte recovery and cumulus attachment, regardless of the needle type. The needle type did not affect the oocyte recovery or cumulus attachment with an optimized vacuum pressure. In the second study, US-guided OPU was performed in live cows using two types of needles with a vacuum pressure of 75 mmHg. The needle type did not affect the oocyte recovery or cumulus attachment of the recovered oocytes. The results revealed that twisting of the needle is effective in follicle aspiration, and suggested that a single-lumen needle is as useful as a double-lumen needle for US-guided OPU in cattle.

【 授权许可】

Unknown