Hydrolytic activities of small intestine content as determined by radial enzyme diffusion in Eurytrema sp infected or non infected cattle

Ciência Rural

Assis, Carlos Batista de²  Belém, Pacífico Antônio Diniz¹  Santana, Marcelo Lopes de² 

[1] Universidade Federal de Viçosa, Viçosa;Universidade Federal de Viçosa

关键词: Eurytrema;    pancreatic function;    fecal hydrolases;    cattle;   

DOI  :  10.1590/S0103-84782000000200026

学科分类：农业科学（综合）

来源: Universidade Federal de Santa Maria * Centro de Ciencias Rurais

【 摘 要 】

Hidrolitic activities of small intestine content as determined by radial enzyme diffusion in agar gel containing calcium caseinate, corn oil, starch or skimmed milk were measured in 38 mature cattle from slaughterhouse. Each one were identified as normal or infected and then classified into two different grades according to pancreatic ducts lesions and parasite burden. The substrates calcium caseinate and corn oil did not shown cleared zones in several radial enzyme diffusion tests or it was difficult to get a correct measure of the diameters. Such problems, however, were not observed with starch and skimmed milk. In these cases, hidrolitic activities were similar in both control and Eurytrema sp infected groups, despite of the pancreatic duct lesions and parasite load (P>0.05). So, it was concluded that the radial enzyme diffusion method couldnÂ’t shown any exocrine pancreatic disfunction in Eurytrema sp infected cattle.

【 授权许可】

CC BY-NC