Evaluation of response to restraint stress by salivary corticosterone levelsin adult male mice

Journal of Veterinary Medical Science

Hiromi AMAO¹  Atsushi TOHEI¹  Takumi SATO¹  Masakatsu NOHARA¹ 

[1] Laboratory of Experimental Animal Science, Nippon Veterinary and Life Science University, 1�?7�?1 Kyonan-cho, Musashino, Tokyo 180�?8602, Japan

关键词: less invasive;    mixed anesthetic agents;    restraint stress;    saliva;    the three Rs;   

DOI  :  10.1292/jvms.15-0610

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(30)Saliva as a sampling method is a low invasive technique for the detection ofphysiologically active substances, as opposed to sampling the plasma or serum. In thisstudy, we obtained glucocorticoids transferred from the blood to the saliva from micetreated with 2.0 mg/kg via an intraperitoneal injection of cortisol. Next, to evaluate theeffect of restraint stress using mouse saliva—collected under anesthesia by mixedanesthetic agents—we measured plasma and salivary corticosterone levels at 60 min afterrestraint stress. Moreover, to evaluate salivary corticosterone response to stress in thesame individual mouse, an adequate recovery period (1, 3 and 7 days) after anesthesia wasexamined. The results demonstrate that exogenous cortisol was detected in the saliva andthe plasma, in mice treated with cortisol. Restraint stress significantly increasedcorticosterone levels in both the plasma and saliva (P<0.001).Monitoring the results of individual mice showed that restraint stress significantlyincreased salivary corticosterone levels in all three groups (1-, 3- and 7-day recovery).However, the statistical evidence of corticosterone increase is stronger in the 7-dayrecovery group (P<0.001) than in the others(P<0.05). These results suggest that the corticosterone levels insaliva reflect its levels in the plasma, and salivary corticosterone is a useful,less-invasive biomarker of physical stress in mice. The present study may contribute toconcepts of Reduction and Refinement of the three Rs in small animal experiments.

【 授权许可】

Unknown