Decontamination of naturally contaminated liquid nitrogen storage tanks

Revista Brasileira de Zootecnia

Silva, Carlos Antonio Mondino¹  Rubin, Mara Iolanda Batistella¹  Rosa, Denize Costa da³  Pessoa, Gilson Antonio² 

[1] Universidade Federal de Santa Maria, Santa Maria, Brasil;Universidade Federal do Rio Grande do Sul, Porto Alegre, Brasil;Universidade de Ijuí, Ijuí, Brasil

关键词: artificial insemination;    bacteria;    fungi;    semen;   

DOI  :  10.1590/S1516-35982014000500004

学科分类：兽医学

来源: Universidade Federal de Vicosa

【 摘 要 】

The objective of this study was to evaluate the efficacy of cleaning and decontamination procedures in liquid nitrogen tanks. We evaluated 151 canisters and 133 bottoms from 133 nitrogen tanks of companies or farms for the presence of bacteria and fungi. Samples were collected from the canisters and the bottom of tanks containing liquid nitrogen. Tanks were divided into Group 1 (G1): tanks decontaminated with 2% glutaraldehyde - Glutaron® II (n = 16 canisters in 8 tanks); Group 2 (G2): decontamination with 70% ethanol (n = 20 canisters in 10 tanks); and Group 3 (G3): decontamination with 70% ethanol (n = 115 canisters in 115 tanks). Tanks in Groups 1 and 2 belonged to companies; Group 3 tanks belonged to farms. The culture of canisters showed twelve genera of bacteria and five genera of fungi. Bacillus cereus was the most prevalent bacterial contaminant (42/133) in liquid nitrogen tanks (31.57%). Decontamination by 2% glutaraldehyde plus 70% ethanol was effective and no difference was found between the decontamination methods of Groups 1 and 2. In Group 3 the decontamination method was considered effective. Handling procedures with high hygienic standards should be recommended to avoid contamination of liquid nitrogen tanks on farms.

【 授权许可】

Unknown