Generation of Monoclonal Autoantibodies from Babesiarodhaini-Infected Mice

Journal of Veterinary Medical Science

Ikuo IGARASHI²  Katsuya KITOH¹  Yasuhiro TAKASHIMA¹  Shih-Pin CHIOU¹ 

[1] United Graduate School of Veterinary Science, Gifu University, 1�?1 Yanagido, Gifu 501�?1193, Japan;National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada-cho, Obihiro, Hokkaido 080�?8555, Japan

关键词: autoantibody;    Babesiosis;    erythrocyte antigen;    immune-mediated anemia;    monoclonal antibody;   

DOI  :  10.1292/jvms.14-0095

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(20)The presence ofanti-erythrocyte autoantibodies in animals infected with various Babesiaspecies is well reported. However, the pathogenesis of autoantibodies in babesiosis ispoorly understood. Here, we demonstrated that anti-erythrocyte immunoglobulin (Ig) M andIgG were present in B. rodhaini-infected mice at 6 and 8 days afterinfection, respectively. Furthermore, we generated monoclonal antibodies againsterythrocyte antigen from B. rodhaini-infected mice. Five clones weregenerated. By Western blotting analysis using whole erythrocyte antigens, one clonereacted with a broad band around 90�?150 kDa, and the 2 clones reacted with a band largerthan 150 kDa. B. rodhaini-infected mice and/or autoreactive monoclonalantibodies established in this study might be a powerful tool for in vivopathogenesis studies of autoantibody development in infectious diseases.

【 授权许可】

Unknown