Sea bass Dicentrarchus labrax nervous necrosis virus isolates with distinct pathogenicity tosea bass larvae

Diseases of Aquatic Organisms

G. Breuil¹  J. F. Pepin¹  O. Mouchel¹  C. Fauvel¹ 

关键词: Fish encephalopathy;    Nodavirus;    ELISA;   

DOI  :  10.3354/dao045025

学科分类：生物科学（综合）

来源: Inter-Research

【 摘 要 】

ABSTRACT: Reproduction of nodavirus disease was performed by experimental infection of sea bass eggs during fertilization or at larval stage 4 with 2 genetically distinguishable nodavirus strains (Sb1 and Sb2) isolated from sea bass collected along theAtlantic and Mediterranean French coast. The pathogenicity of the virus strains was assigned after detection of the virus by ELISA and immunohistochemistry (IHC). The Atlantic (Sb1) strain was more pathogenic than the Mediterranean (Sb2) strain during thefertilization step whilst both strains were pathogenic following experimental exposure of 4 d old larvae. Virus lesions developed in the brain 4 to 6 d following experimental exposure. Experimental ELISA proved very sensitive for detecting the nodavirusin Sb1 or Sb2 experimentally infected larvae, as well as in naturally infected sea bass larvae collected in French hatcheries or in barramundi larvae reared in the Pacific area. The development of an ELISA specific for the 2 nodavirus strains isolatedfrom the sea bass should be useful for the detection of the virus, in addition to other techniques recommended by the Office International des Epizooties (OIE).

【 授权许可】

Unknown