P-Glycoprotein-Mediated Efflux and Drug Sequestration in Lysosomes Confer Advantages of K562 Multidrug Resistance Sublines to Survive Prolonged Exposure to Cytotoxic Agents | Science Publications

American Journal of Applied Sciences

Samlee Mankhetkorn¹  Nathupakorn Dechsupa¹ 

关键词: Multidrug Resistance (MDR);    P-glycoprotein (Pgp);    Acridine Orange (AO);    Drug sequestration in lysosomes;    Pirarubicin;   

DOI  :  10.3844/ajassp.2009.1637.1646

学科分类：自然科学（综合）

来源: Science Publications

【 摘 要 】

Problem statement: Cellular drug resistance to anticancer agents is major obstacle in cancer chemotherapy and the mechanisms by which these MDR cells possess for protecting themselves to survive prolonged exposure to cytotoxic agents still debating. The study aimed to clarify the role of P-glycoprotein (Pgp) and enhanced drug sequestration in lysosomes to confer the multidrug resistance K562 cells with varied degree of Pgp expression. Approach: Erythromyelogenous leukemic K562 and its corresponding Pgp-over expression K562/adr (RF = 26.5) and K562/10000 (RF = 39.6) cells were used. The transport of intrinsic fluorescence molecules including acridine orange and pirarubicin across plasma membrane of living cells was performed by using spectrofluorometric and flow cytometric analysis. Results: Pirarubicin passively diffused through the plasma membrane of K562, K562/adr and K562/10000 cells with the same values of k+ = 3.4

【 授权许可】

Unknown