Further Development of a Recombinant Feline Herpesvirus Type 1 Vector Expressing Feline Calicivirus Immunogenic Antigen

Journal of Veterinary Medical Science

Kentaro FUJITA³  Masami MOCHIZUKI¹  Takeshi MIKAMI³  Ken MAEDA²  Sinryo ISHIGURO¹  Kyoko KUROSAWA³  Naoaki YOKOYAMA³  Takayuki MIYAZAWA³  Armando DAMIANI³  Eiji SATO³ 

[1] Tsukuba Laboratories, Kyoritsu Shoji Co., Ltd.;Department of Veterinary Microbiology, Faculty of Agriculture, Yamaguchi University;Department of Veterinary Microbiology, Faculty of Agriculture, The University of Tokyo

关键词: feline calicivirus;    feline herpesvirus type 1;    recombinant polyvalent vaccine;   

DOI  :  10.1292/jvms.60.717

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(29)Cited-By(7)We previously reported the attenuation of thymidine kinase (TK) deficient mutant (C7301dlTK) of feline herpesvirus type 1 (FHV-1) in cats and the construction of a recombinant FHV-1 (C7301dlTK-Cap) inserted a precursor capsid gene of feline calicivirus (FCV) into the TK deletion locus of the C7301dlTK. In this study, we constructed a further improved recombinant FHV-1 (dlTK(gCp)-Cap) carrying a putative FHV-1 gC promoter sequence upstream of the FCV precursor capsid gene of the C7301dlTK-Cap. Growth kinetics of the dlTK(gCp)-Cap in cell cultures was similar to those of C7301dlTK and C7301dlTK-Cap. A strong expression of FCV immunogenic antigen by dlTK(gCp)-Cap was confirmed by indirect immunofluorescence and enzyme-linked immunosorbent assays. In addition, one vaccination with dlTK(gCp)-Cap protected cats more effective against subsequent virulent FCV challenge than that with C7301dlTK-Cap.

【 授权许可】

Unknown