Antibiotic Resistance in Staphylococcus aureusStrains Isolated from Cows with Mastitis in Eastern Poland and Analysis of Susceptibilityof Resistant Strains to Alternative Nonantibiotic Agents: Lysostaphin, Nisin and PolymyxinB

Journal of Veterinary Medical Science

Marta SCHIELMANN¹  Barbara KOT²  Magdalena ZALEWSKA²  Piotr SZWEDA¹  Aneta FRANKOWSKA² 

[1] Department of Pharmaceutical Technology and Biochemistry, Faculty of Chemistry, Gdańsk University of Technology, G. Narutowicza 11/12, 80�?233 Gdańsk, Poland;Department of Microbiology, Institute of Biology, Siedlce University of Natural Sciences and Humanities, Bolesława Prusa 12, 08�?110 Siedlce, Poland

关键词: lysostaphin;    mastitis;    nisin;    polymyxin B;    Staphylococcus aureus;   

DOI  :  10.1292/jvms.13-0177

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(42)Cited-By(11)The aim of this study was to analyze the resistance of Staphylococcusaureus isolates from bovine mastitis in the eastern part of Poland to a set of20 antibiotics and three alternative agents: lysostaphin, nisin and polymyxin B.Eighty-six out of 123 examined isolates were susceptible to all 20 tested antibiotics(70%). The highest percentage of resistance was observed in the case of β-lactamantibiotics: amoxicillin (n=22, 17.9%), ampicillin (n=28, 22.8%), penicillin (n=29, 23.6%)and streptomycin (n=13; 10.6%). Twenty-five of the penicillin-resistant strains were foundto carry the blaZ gene coding for β-lactamases. Two strains were found tobe mecA positive and a few strains were classified as multidrug resistant(MDR), one of them was simultaneously resistant to six antibiotics. All strains, resistantto at least one antibiotic (n=37) and two control strains, were susceptible to lysostaphinwith MIC values of 0.008�?0.5 µg/ml (susceptibilitybreakpoint 32 µg/ml). Twenty-one (54%) isolates weresusceptible to nisin. The MIC value of this agent for 17 (44%) strains was 51.2µg/ml and was not much higherthan the susceptibility breakpoint value (32 µg/ml).Polymyxin B was able to inhibit the growth of the strains only at a high concentration(32�?128 µg/ml). The presented results confirmed theobserved worldwide problem of spreading antibiotic resistance among staphylococci isolatedfrom bovine mastitis; on the other hand, we have indicated a high level of bactericidalactivity of nisin and especially lysostaphin.

【 授权许可】

Unknown