Xenotransplantation of Porcine Pancreatic Endocrine Cells to Total Pancreatectomized Dogs

Journal of Veterinary Medical Science

Kazuya EDAMURA³  Koko NASU³  Yukiko IWAMI³  Hiroyuki OGAWA²  Shin ITAKURA¹  Nobuo SASAKI¹  Hisako OHGAWARA³ 

[1] Laboratory of Veterinary Surgery, Graduate School of Agricultural and Life Sciences, The University of Tokyo;Laboratory of Veterinary Emergency Medicine, Graduate School of Agricultural and Life Sciences, The University of Tokyo;Division of Cell Replacement and Regenerative Medicine, Medical Research Institute, School of Medicine, Tokyo Women's Medical University

关键词: bio-artificial pancreas;    canine;    islet;    swine;    xenotransplantation;   

DOI  :  10.1292/jvms.65.549

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(41)Cited-By(11)Xenotransplantation of porcine pancreatic endocrine (PE) cells in a diffusion chamber, a bioartificial endocrine pancreas (Bio-AEP), was conducted to total pancreatectomized dogs. Six pancreatectomized dogs were divided into two groups of 3 dogs each. In three dogs of the control group, exogenous insulin was administered twice a day for 30 weeks to maintain fasting blood glucose (FBG) levels within the normal range. The remaining three dogs were implanted with Bio-AEPs (implantation group), in addition to daily insulin administration. In the implantation group, Bio-AEPs containing 1.3 to 1.8 × 107 cells per kg of body weight of the recipient were implanted without fixation into the abdominal cavity. In the control group, exogenous insulin requirements did not decrease during the experimental period, whereas it significantly decreased for a certain period (3, 11, 17 weeks) after implantation in all implanted dogs. In the implantation group, laparotomy was performed after FBG and the exogenous insulin requirement increased again and Bio-AEPs were removed. Two Bio-AEPs were completely destroyed, and the remaining one was encapsulated by thin fibrous tissue. In this dog, effusion was present within the capsule, but the Bio-AEP was not destroyed. Histopathologically, the necrosis, presumably caused by hypoxia, of the PE-cells was observed on transmission electron microscopy. In conclusion, Bio-AEP could function for a certain period after implantation in this study. However, more preclinical researches should be needed to apply this technique for the treatment of diabetic dogs.

【 授权许可】

Unknown