High-Temperature, but Not High-Pressure, Conditions Alter Neuronal Activity

Journal of Pharmacological Sciences

Naoya Takahashi¹  Yuji Ikegaya¹  Mika Mizunuma¹  Atsushi Usami¹  Norio Matsuki¹ 

[1] Laboratory of Chemical Pharmacology, Graduate School of Pharmaceutical Sciences, The University of Tokyo, Japan

关键词: pressure;    calcium imaging;    neuronal network;   

DOI  :  10.1254/jphs.09031SC

学科分类：药学

来源: Nihon Yakuri Gakkai Henshuubu / Japanese Pharmacological Society

【 摘 要 】

References(14)Cited-By(5)We describe the effect of high pressure and high temperature on neuronal activity. Increased intracranial pressure is generally a pathological sign observed in intracerebral hemorrhage, brain edema, and brain tumor, yet little is known about how the hyperbaric pressure per se affects neuronal activity. Using a pressure/temperature-changeable perfusion chamber, we carried out functional multineuron calcium imaging to record spontaneous spiking activity simultaneously from about 100 neurons in hippocampal slice cultures. High-pressure conditions (up to 100 mmHg) did not alter the network excitability, whereas high-temperature conditions (up to 40°C) increased synchronized network activity. Thus, neurons are sensitive to feverish conditions, but the acute hyperbaric circumstance itself is unlikely to exert a detrimental effect on neuronal function.

【 授权许可】

Unknown