Evaluation of Peripheral Metabolic Status by Determination of Na-K ATPase Pump Activity in Circulating Erythrocytes in Patients with Thyroid Diseases and Nonthyroidal Illnesses

Endocrine Journal

HIDENORI OGASAWARA¹  MITSUSHICE NISHIKAWA¹ 

[1] Second Department of Internal Medicine, Kansai Medical University

关键词: Nonthyroidal illness;    Na-K ATPase;    Erythrocyte;    Metabolic status;    Maximal ouabain binding;   

DOI  :  10.1507/endocrj.40.27

学科分类：内分泌与代谢学

来源: Japan Endocrine Society

【 摘 要 】

References(14)Cited-By(4)The number of Na-K ATPase units in erythrocytes (RBC) was determined by the maximal ouabain binding assay in 25 normal subjects and patients with hyperthyroidism (n=29), hypothyroidism (8), chronic renal failure (CRF, 19) and with neoplastic disorders (NP, 12). The activity of the pump units was also assessed by measuring ouabain-sensitive 86Rb uptake in some of these subjects. In addition, it was determined in mononuclear cells in normal controls and patients with hyper- and hypothyroidism and CRF. Significant diminution of the number of the RBC pump units was found in hyperthyroidism, while it was increased in hypothyroidism. The binding (O) of old RBC was significantly lower than that (Y) of young RBC and a striking correlation was observed between the % reduction rate ((Y-O)/Y) of the binding and the serum T4 level in hyperthyroidism (r=0.85, P<0.02). No difference was observed in pump units of mononuclear cells in normal and hyper- and hypothyroidism. It is suggested that the thyroid hormone- mediated disappearance of the pump units in RBC may play a role in reducing the number of pump units in RBC in hyperthyroidism. The ratio of RBC 86Rb uptake to the number of the pump units in the same cell (U/B) bore a significant relation to serum T3(r=0.48, P<0.05) and T4 (r=0.49, P<0.05) indicating that the U/B is a useful index for the peripheral metabolic status. In CRF patients with low T3 levels, bindings were increased but those in NP with low T3 was almost normal. The increased bindings were observed in NP patients with normal T3. These findings suggest that the metabolic status in CRF and NP patients could not be always assessed by the number of the ouabain sensitive Na-K ATPase pump units. However, the U/B was almost normal in all patients with CRF and NP, suggesting that pump activity as a measure of oxygen consumption and thermogenesis may not be in the hypothyroid state in patients with nonthyroidal illnesses.

【 授权许可】

Unknown