Effects of BST and high energy diet on gene expression in mammary parenchyma of dairy heifers

Revista Brasileira de Zootecnia

Rennó, Francisco Palma³  Carvalho, Marina Vieira de³  Freitas Júnior, José Esler de³  Lew, Betina Joyce¹  Oliveira, Mauro Dal Secco de²  Rêgo, Aníbal Coutinho do² 

[1] Michigan State University, East Lansing, USA;Universidade Estadual Paulista Júlio de Mesquita Filho, Jaboticabal, Brazil;Universidade de São Paulo, Pirassununga, Brazil

关键词: gene regulation;    mammary gland development;    microarray;    nutrition;    somatotropin    Introduction The establishment of milk yield potential is critically determined during the prepubertal phase of mammary development (Sejrsen & Purup;    1997);   

DOI  :  10.1590/S1516-35982013000700008

学科分类：兽医学

来源: Universidade Federal de Vicosa

【 摘 要 】

The objective of this study was to determine the effects of dietary energy and recombinant bovine somatotropin (bST) injection to identify genes that might control mammogenesis. Total RNA was extracted from the parenchymal tissue of 32 heifers randomly assigned to one of four treatments: two diets (a standard diet and a high energy, high protein diet), each with or without bST. To perform microarray experiments, RNA samples were pooled (2 animals/pool) before reverse transcription and labeling with Cy3 or Cy5. A 4-node loop design was used to examine the differential gene expression among treatments using a bovine-specific cDNA microarray (National Bovine Functional Genomics Consortium Library, NBFGC) containing 18,263 unique expressed sequence tags (EST). Significance levels of differential gene expression among treatments were assessed using a mixed model approach. Injection of bST altered the expression of 12 % of the genes on NBFGC slide related to tissue development, whereas 6% were altered by diet. Administration of bST increases the expression of genes positively related to cell proliferation and mammary parenchyma to a greater extent than a high energy diet.

【 授权许可】

CC BY-NC