Interactomic analysis of VDAC (voltage-dependent anion selective channel) in rat, bovine and chicken brain

Pesquisa Veterinária Brasileira

Munin, Flávia Simone¹  César, Marcelo de Cerqueira¹  Vitale, Phelipe Augusto Mariano¹  Crepaldi, Carla Rossini¹ 

[1] Universidade de São Paulo, Pirassununga, Brazil

关键词: VDAC;    Hexokinase;    interactome;    epilepsy;    mitochondria;    neuron;    brain;   

DOI  :  10.1590/S0100-736X2011001100016

来源: Colegio Brasileiro de Patologia Animal-CBPA

【 摘 要 】

The voltage dependent anion channel (VDAC) is the most abundant protein of outer mitochondrial membrane. VDAC controls metabolite exchange through this membrane and the apoptosis machinery. Interactomics is the study of protein complexes, their interactions and the consequences of these interactions. In our case we studied the interactome of the hexokinase-VDAC-ANT (adenine nucleotide transporter) complex in mitochondria of neuronal cells from rat, bovine and chicken brain. We wished to understand if the differential expression of VDAC1 and VDAC2 verified in these cells was linked to differences in the interactions between proteins in these complexes. Our results showed that avian and bovine neurons had more protein complexes (5) containing VDAC than rat cells (1), which indicates a differential kinetics of assembly or disassembly. Moreover, mitochondrial neuronal chicken VDAC interacts with more proteins in comparison with bovine neuronal VDAC, which is indicative of a differential subunit composition. These results supported evidences of differential apoptotic and energetic mechanisms between these brains.

【 授权许可】

CC BY-NC