Analysis of Cartilage Oligomeric Matrix Protein (COMP) in Synovial Fluid, Serum and Urine from 51 Racehorses with Carpal Bone Fracture

Journal of Veterinary Medical Science

Koh ARAI³  Yuri SHIMIZU³  Kazuhiro MISUMI³  Takashi HATAZOE¹  Masaaki TAGAMI²  Eikoh NISHIMATSU³  Makoto FUJIKI³ 

[1]The Japan Bloodhorse Breeder's Association Kyusyu Stallion Station

[2]Shadai Horse Clinic

[3]Department of Veterinary Clinical Science, Kagoshima University

关键词: COMP;    equine;    fracture;    osteoarthritis;    urine;   

DOI  :  10.1292/jvms.70.915

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(20)Cited-By(7)We investigated the relationship between cartilage oligomeric matrix protein (COMP) levels in synovial fluid (SF), serum and urine and the development of osteochondral damage and osteophyte (OP) formation following intraarticular fractures of the carpus in racehorses in order to assess the clinical usefulness of COMP as a diagnostic biomarker of developmental osteoarthritis (OA). Two monoclonal antibodies (mAb clones 2A11 and 3C8) raised against equine COMP were shown to be capable of detecting the molecule in serum and urine as well as SF. Fifty-one samples were obtained from 26 OP-positive (OP(+)) and 25 OP-negative (OP(-)) racehorses with carpal bone fracture, in whom OP was ascertained arthroscopically and radiographically. The COMP measurements obtained using the two mAbs were highly correlated with each other in SF, serum, or urine. Horses with OP(+) showed a significantly higher [urinary COMP (μg)]/[urinary creatinine (mg)] ratio (4.94 ± 5.10 and 1.46 ± 1.19, using mAbs 2A11 and 3C8, respectively) than OP(-) horses (2.80 ± 1.72 and 0.93 ± 0.49, respectively). The relationship between serum and urine COMP levels and the period from injury to surgery were extrapolated using a polynomial expression. Measurement of COMP, especially in urine, has potential as a predictive marker of advanced OA following carpal bone fractures in racehorses.

【 授权许可】

Unknown