Molecular characterization of a ranavirus isolated from largemouth bass Micropterus salmoides

Diseases of Aquatic Organisms

Jun Wang¹  Giovina D. Chinchar¹  Jinghe Mao¹  V. Gregory Chinchar¹ 

关键词: Largemouth bass;    Micropterus salmoides;    Ranavirus;    Iridovirus;    Fish die-off;    Viral taxonomy;   

DOI  :  10.3354/dao037107

学科分类：生物科学（综合）

来源: Inter-Research

【 摘 要 】

ABSTRACT: An iridovirus, isolated from largemouth bass Micropterus salmoides following a die-off among adult fish and provisionally designated largemouth bass virus (LMBV), was characterized by analysis of viral protein synthesis in infected cells,viral DNA restriction fragment length polymorphisms (RFLP), and sequence determination of the major capsid protein and viral DNA methyltransferase genes. All 3 approaches yielded results consistent with the suggestion that LMBV was a member of the genusRanavirus. Moreover, LMBV was nearly identical to 2 isolates from Southeast Asia which had been previously detected in imported ornamental fish. It remains to be determined whether infection of largemouth bass resulted from exposure to an importedvirus, or whether the presence of similar viruses in southeast Asia and the southeastern United States indicates that iridovirus species are not geographically limited as suggested earlier, but rather globally distributed.

【 授权许可】

Unknown