Genetic characterization of the lobster pathogen Aerococcus viridans var. homari by 16S rRNA gene sequence and RAPD

Diseases of Aquatic Organisms

Richard J. Cawthorn¹  Spencer J. Greenwood¹  Béatrice M. Després¹  Ian R. Keith¹ 

关键词: Lobster;    Homarus americanus;    Gaffkemia;    Aerococcus viridans var. homari;    RAPD;    16S rRNA;   

DOI  :  10.3354/dao063237

学科分类：生物科学（综合）

来源: Inter-Research

【 摘 要 】

ABSTRACT: A combination of 16S rRNA sequencing and random amplified polymorphic DNA (RAPD) analysis was used to evaluate the genetic diversity within Aerococcus viridans var. homari, the causative agent of gaffkemia in lobsters. A collectionof 7 A. viridans var. homari strains and 2 avirulent A. viridans-like cocci isolated from homarid lobsters harvested from different regions on the Atlantic Coast of North America were analyzed. The isolates are separatedgeographically and temporally between the years 1947 and 2000. Sequencing of 16S rRNA genes confirmed the inclusion of all 9 isolates in the monophyletic A. viridans clade (99.8 to 100% similarity). RAPD analysis revealed that the 9 A. viridansvar. homari isolates could be separated into 2 distinct subtypes. Subtype 1 included the 7 pathogenic lobster isolates and constituted a homogeneous group regardless of their geographical, temporal or virulence differences. Subtype 2 containedthe 2 avirulent A. viridans-like cocci that had distinct RAPD patterns and clustered separately with the non-marine A. viridans. RAPD analysis represented a useful method for determining molecular subtyping for the intraspecificclassification and epidemiological investigations of A. viridans var. homari.

【 授权许可】

Unknown