Bacterial diversity and community structure in acid mine drainage from Dabaoshan Mine, China

Aquatic Microbial Ecology

Xue-duan Liu¹  Guan-zhou Qiu¹  Hong Peng¹  Ji-zhong Zhou¹  Yu Yang¹  Min-xi Wan¹  Wu-yang Shi¹ 

关键词: Bacterial diversity;    Microbial community;    Acid mine drainage;    Amplified ribosomal DNA restriction analysis;   

DOI  :  10.3354/ame047141

学科分类：生物科学（综合）

来源: Inter-Research

【 摘 要 】

ABSTRACT: Three samples of acid mine drainage (AMD) collected from Dabaoshan Mine (Guangdong Province, China) were studied. In addition to physico-chemical analyses, bacterial diversities and community structures of these samples were described at genetic level by amplified ribosomal DNA restriction analysis (ARDRA). A total of 60 different ARDRA patterns were obtained from 377 clones and were studied as operational taxonomic units (OTUs), which were re-amplified and sequenced. The sequence data and phylogenetic analysis showed that Acidithiobacillus ferrooxidans represented 88.0% of the bacterial population in Sample LC. However, Samples JX and FS contained more diverse colonies of bacteria, such as Leptospirillum ferrooxidans (JX: 16.9%, FS: 39.1%), Acidiphilum sp. (JX: 38.7%, FS: 25.8%) and A. ferrooxidans (JX: 12.1%, FS: 10.2%). These diversities were characterized by the reciprocal of Simpson’s index (1/D) and correlated with the concentrations of ferrous iron and toxic ions in AMD.

【 授权许可】

Unknown