期刊论文详细信息
Diseases of Aquatic Organisms
Detection and characterization of Bonamia ostreae in Ostrea edulis imported to China
Jizhou Lv1  Caixia Wang1  Chunyan Feng1  Xiangmei Lin1  , Huailin Li1  Lin Mei1  Shaoqiang Wu1  Yongning Zhang1  Fei Wang1  Junhua Deng1 
关键词: Bonamia ostreae;    Ostrea edulis;    In vitro culture characterization;    Fluorescent in situ hybridization;    Transmission risk;   
DOI  :  10.3354/dao02631
学科分类:生物科学(综合)
来源: Inter-Research
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【 摘 要 】

ABSTRACT: The protozoan parasite Bonamia ostreae is a destructive pathogen of flat oysters and has been reported to be widespread in Europe and North America. The biological characteristics of this unicellular parasite are still not fully understood. In this study, 104 Ostrea edulis imported from the USA to the Guangdong province of China for consumption were examined for Bonamia infection. PCR assay, combined with restriction fragment length polymorphism, sequencing and BLAST analysis, showed that B. ostreae DNA could be detected in 1 of the 104 oyster samples. Light microscopy revealed Bonamia-like organisms in the oyster. PCR assay and fluorescent in situ hybridization showed that B. ostreae organisms were present and retained their integrity after 4 wk in culture. Acridine orange-ethidium bromide staining indicated that the B. ostreae were still alive. In conclusion, B. ostreae was present in oysters imported to China. More importantly, the parasite was able to survive for at least 4 wk of in vitro culture at 4°C, which further implied a long-term transmission risk of B. ostreae. Considering the wide culture beds of Crassostrea ariakensis and C. gigas in China, and that C. ariakensis and C. gigas are susceptible hosts or reservoirs of B. ostreae, our study highlights the potential risk of introducing B. ostreae by importing O. edulis from a Bonamia endemic area.

【 授权许可】

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