Molecular Analysis of Endogenous Avian Leukosis/Sarcoma Virus Genomes in Korean Chicken Embryos

Journal of Veterinary Medical Science

Sang-Ik PARK³  Byoung-Gui JANG¹  Hak-Kue KIM¹  Ha-Hyun KIM³  Yong-Wun JUNG²  You-Jung KIM³  Kyoung-Oh CHO³  Su-Jin PARK³  Jung-Taek KWON⁴ 

[1] The Poultry Research Division, National Live Stock Research Institute;The Intervet Korea Ltd.;Bio-Therapy Human Resources Center, College of Veterinary Medicine, Chonnam National University;The Halim Ltd.

关键词: avian leukosis/sarcoma virus;    chickens;    genetic divergence;    prevalence;   

DOI  :  10.1292/jvms.70.17

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(21)Since the status of endogenous avian leucosis/sarcoma virus (ALSV) infections in Korean broiler chickens is unclear, this study examined embryonated eggs obtained from broiler farms and Korean native chicken breeds in Korea using PCR with the primer sets specific for endogenous ALSVs. The PCR assays detected the genomes of EAV, ev, ev/J and ART-CH belonging to the endogenous ALSV from all embryos tested. Phylogenetically, the Korean EAV genomes were more closely related to the prototype EAV-0 than to the other prototype, E51. The Korean ART-CH elements clustered together but were distinct from the prototype ART-CH clones, 5 and 14. Although there was comparatively little divergence in the nucleotide and amino acid sequences of the Korean ev and ev/J genomes compared with the other known ev and ev/J genomes, the Korean genomes had phylogenetically distinct branches. From these results, endogenous genomes are quite prevalent in Korean broiler chickens. In addition, the endogenous genomes circulating in Korean broiler chickens are genetically different from the other known endogenous genomes. These results are expected to provide useful information for the control and establishment of a surveillance system for endogenous ALSVs in Korea.

【 授权许可】

Unknown