Differences of Cell Growth and Cell Cycle Regulators Induced by Basic Fibroblast Growth Factor Between Nifedipine Responders and Non-responders

Journal of Pharmacological Sciences

Reiri Takeuchi¹  Yoshiaki Akimoto²  Hidehiko Okada¹  Akihiko Gunji¹  Kosuke Hakozaki¹  Akira Fujii¹  Hiroko Matsumoto¹  Mami Hori¹ 

[1] Department of Oral Molecular Pharmacology, Nihon University School of Dentistry at Matsudo, Japan;Department of Oral Surgery, Nihon University School of Dentistry at Matsudo, Japan

关键词: gingival overgrowth;    nifedipine;    gingival fibroblast;    cell cycle;   

DOI  :  10.1254/jphs.FP0060928

学科分类：药学

来源: Nihon Yakuri Gakkai Henshuubu / Japanese Pharmacological Society

【 摘 要 】

References(32)Cited-By(11)Differences of cell proliferation, cell cycle, and G1/S transition regulatory proteins of gingival fibroblasts derived from nifedipine-reactive patient (NIFr) and nifedipine-non-reactive patient (NIFn) in the presence of basic fibroblast growth factor (bFGF) were investigated to elucidate the mechanism of gingival overgrowth associated with nifedipine, one of the Ca2+-channel blockers. The proliferation rate of NIFr cells in the presence of bFGF significantly increased than NIFn cells. The proportion of NIFr cells that had undergone progression to the S and G2/M phases from the G0/G1 phase significantly increased compared to that in NIFn cells. Increases of pRB (Ser807/811), pCDK2 (Thr160), CDK2, and cyclin E protein levels in NIFr cells were greater than those in NIFn cells. The elevations of pRB (Ser780), RB, and cyclin A protein levels in NIFr cells did not differ from those of NIFn cells. The growth of NIFr cells was greater than that of NIFn cells as a result of the active G1/S transition of NIFr cells, as assessed by the increments of cyclin E, pCDK2, and pRB (ser807/811) protein in NIFr cells.

【 授权许可】

Unknown